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Principal Investigator Research Topics
Bicciato Silvio

BIOINFORMATICS.

Ongoing research projects:

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  • Analysis of high-throughput genomic data from RNA-seq, ChIP-seq, Exome-sequencing, methylation, microarrays, and protein arrays
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  • Genome-wide definition of regulatory elements in physiological and pathological cell states
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Bortolotti Carlo Augusto

DEVELOPMENT OF BIO-(IN)ORGANIC INTERFACES FOR NANOBIOTECHNOLOGICAL APPLICATIONS (biosensors, smart surfaces)

DYNAMICS/FUNCTION RELATIONSHIP IN HEALTH-RELEVANT METALLOPROTEINS

De Luca Michele

MOLECULAR MECHANISMS REGULATING EPITHELIAL STEM CELL RENEWAL AND PROLIFERATION AND EPITHELIAL STEM CELL-MEDIATED CELL AND GENE THERAPY.

BASIC RESEARCH

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  • Molecular and functional characterization of human epithelial stem cells (epidermis, cornea, conjunctiva, urethra, oral mucosa and airway epithelia).
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  • studies of molecular mechanisms regulating self-renewal, proliferation and differentiation of squamous epithelial stem cells by conventional methods integrated with genomic (genotyping and next generation sequencing), epigenomic and transcriptomic technologies.

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CELL THERAPY

Definition of experimental and clinical protocols aimed at clinical application of:

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  • epidermal stem cells in different cutaneous pathologies;

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  • limbal stem cells in different pathologies of the ocular surface;

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  • stem cells of conjunctival, urethral and buccal epithelia in traumatic destruction of the conjunctival epithelium, dry-eye Syndrome, posterior hypospadias, recurring urethral stenosis, reconstructive surgery of oral cavity;
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  • stem cells of respiratory epithelia for the reconstruction of upper respiratory tract.
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GENE THERAPY

Definition of experimental and clinical protocols for ex-vivo gene therapy of laminin 5-dependent and Collagen XVII-dependent Junctional Epidermolysis Bullosa and Collagen VII-dependent Dystrophic Epidermolysis Bullosa by new generation SIN-retroviral vectors and/or homologous recombination technology.

Dominici Massimo  
Fanelli Francesca

COMPUTATIONAL STRUCTURAL BIOLOGY

Ongoing projects

  • Structural characterization of rhodopsin mutants associated with the Autosomal Dominant form of Retinitis Pigmentosa (ADRP);
  • structure-based discovery/development of pharmacological chaperones with therapeutic potential against ADRP caused by rhodopsin mutations;
  • structure-based discovery/development of inhibitors of Lbc-RhoA interaction with therapeutic potential as anticancer agents;
  • structural characterization of vasopressin 2 receptor (V2R) mutants linked to the Nephrogenic Syndrome of Inappropriate Antidiuresis (NSIAD) and structure-based discovery of V2R ligands as potential therapeutic agents against NSIAD;
  • structure-based discovery of small ligands of the KDEL receptor;
  • unveiling the functional dynamics of RhoA and RhoGEFs of the Dbl family;
  • development and application of the Protein Structure Network (PSN) analysis tool and Web server to unveil the structural communication in biomolecular systems;
  • maintenance and development of the Wordom software for the analysis of molecular structures, trajectories, and free energy surfaces.
Ferrari Sergio

DIFFERENTIATION PLASTICITY IN HUMAN NORMAL HAEMATOPOIETIC STEM CELLS.

Research topics:

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  • Nuclear architecture and chromatin modification in normal human myelopiesis and in leukemic contest
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  • Positional effect of gene expession during normal human myelopiesis
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  • Regulation of gene expression in normal human myelopoiesis
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Imbriano Carol

TRANSCRIPTIONAL AND NON-TRANSCRIPTIONAL CONTROL OF CELL PROLIFERATION AND DIFFERENTIATION.

The activity of the laboratory is focused on NF-Y,  a heterotrimeric transcription factor with a  “pioneering” role in the establishment of transcriptional programs. NF-Y regulates the transcription of several genes, such as cell cycle, pro- and anti-apoptotic genes, whose expression is fundamental in the balance between cell proliferation and cell death. NF-Y is one of the transcription factors orchestrating oncogenic transcriptional changes, and clinical studies correlated upregulated expression of its target genes to poor clinical prognosis in multiple types of cancer.  Moreover, NF-Y has a key role in the control of proliferation and differentiation programs of pluripotent and adult stem cells.

Our research is directed towards investigating the transcriptional and non-transcriptional activity of NF-Y in cancer and muscle stem cells.

To know more about our research projects click here.

Losi Lorena

IDENTIFICATION OF GENETIC AND EPIGENETIC ALTERATIONS IN CANCERS TO SEARCH BIOMOLECULAR MARKERS FOR DIAGNOSIS, PROGNOSIS AND SELECTIVE THERAPIES.  

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  • Comprehension of  mechanism  of chemopreventive agents  in colorectal cancers and characterization of  clinical, pathological and molecular  features related to the altered expression of  implicated proteins
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  • Molecular characterization of the role played by MEF2 factors in the pathogenesis of colorectal cancers
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  • Characterization of molecular bases involved in the development of ovarian tumors
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Luppi Mario

GENETIC AND IMMUNOLOGIC PATHOGENESIS OF HEMATOLOGIC DISEASES.

Special areas of research activity:

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  • The study of genetic and immunologic risk factors for viral and fungal infections in bone marrow/stem cell and solid organ transplantation;
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  • The development and validation of new immunologic tests for the diagnosis and monitoring of infectious agents in at risk immunocompromised patient groups;
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  • The study of anti-tumor immune responses in acute leukemia patients undergoing targeted therapy;
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  • The investigation of new  immuno-therapeutic approaches for virus-related tumors.
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Manfredini Rossella

MOLECULAR AND FUNCTIONAL CHARACTERIZATION OF NORMAL AND CANCER STEM CELLS.

Research Topics:

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  • Gene expression profiling by DNA microarrays of Leukemic Stem Cells (LSC) of myeloproliferative diseases (Chronic Myeloid Leukemia, Primary and Secondary Myelofibrosis, Polycitemia Vera and Essential Thrombocytemia).
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  • Identification of the regulatory mRNA/microRNA networks in Leukemic Stem Cells (LSC) of myeloproliferative diseases.
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  • Molecular and functional characterization of the hematopoietic stem cell niche
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  • Study of the role of myb protooncogene in normal and leukemic differentiation.
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  • Molecular characterization of Epithelial stem cells.
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Marasca Roberto  
Marigo Valeria

MOLECULAR BIOLOGY OF RETINAL DEGENERATION.

The focus of the laboratory is basic and preclinical research on genetic diseases affecting vision: retinal degeneration and albinism. Retinal degenerations are the major cause of incurable blindness during the working age.  The genetic defect has been identified for some of them, however the pathogenetic mechanism causing photoreceptor cell death is still not well understood.

We are currently studying the molecular mechanisms underlying cell death during retinal degeneration and developing molecular and pharmacological approaches to delay and inhibit programmed cell death in the retina. The second line of study is based on retinal derived stem cells. We are currently studying the biology of retinal stem cells dissected from the ciliary body of adult murine and human eyes. We developed protocols to differentiate these cells either into retinal pigment epithelium or into functional rod photoreceptors, the cells affected in retinitis pigmentosa.

Mattioli Anna Vittoria  
Palumbo Carla

HISTO-PHYSIO-PATHOLOGY OF SKELETAL TISSUES AND OF THE ORGANS OF THE DIGESTIVE AND FEMALE REPRODUCTIVE SYSTEMS.

To know the more significant research lines click here.

Pellegrini Graziella

MOLECULAR MECHANISMS REGULATING EPITHELIAL STEM CELL RENEWAL AND PROLIFERATION AND EPITHELIAL STEM CELL-MEDIATED CELL AND GENE THERAPY.

BASIC RESEARCH

  •  
  • Molecular and functional characterization of human epithelial stem cells (epidermis, cornea, conjunctiva, urethra, oral mucosa and airway epithelia).
  •  
  • studies of molecular mechanisms regulating self-renewal, proliferation and differentiation of squamous epithelial stem cells by conventional methods integrated with genomic (genotyping and next generation sequencing), epigenomic and transcriptomic technologies.

  •  

CELL THERAPY

Definition of experimental and clinical protocols aimed at clinical application of:

  •  
  • epidermal stem cells in different cutaneous pathologies;

  •  
  • limbal stem cells in different pathologies of the ocular surface;

  •  
  • stem cells of conjunctival, urethral and buccal epithelia in traumatic destruction of the conjunctival epithelium, dry-eye Syndrome, posterior hypospadias, recurring urethral stenosis, reconstructive surgery of oral cavity;
  •  
  • stem cells of respiratory epithelia for the reconstruction of upper respiratory tract.
  •  

GENE THERAPY

Definition of experimental and clinical protocols for ex-vivo gene therapy of laminin 5-dependent and Collagen XVII-dependent Junctional Epidermolysis Bullosa and Collagen VII-dependent Dystrophic Epidermolysis Bullosa by new generation SIN-retroviral vectors and/or homologous recombination technology.

Pietrangelo Antonello

GENETIC AND MOLECULAR BASIS OF IRON DISORDERS.

Ongoing research projects:

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  • Transcriptional studies on hepcidin regulation in physiology and pathophysiology
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  • The pathogenic basis for hereditary hemochromatosis and the ferroportin disease
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  • The role of iron in the metabolic syndrome, diabetes and cardiovascular diseases
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Potenza Leonardo

GENETIC AND IMMUNOLOGIC PATHOGENESIS OF HEMATOLOGIC DISEASES.

Special areas of research activity:

  •  
  • The study of genetic and immunologic risk factors for viral and fungal infections in bone marrow/stem cell and solid organ transplantation;
  •  
  • The development and validation of new immunologic tests for the diagnosis and monitoring of infectious agents in at risk immunocompromised patient groups;
  •  
  • The study of anti-tumor immune responses in acute leukemia patients undergoing targeted therapy;
  •  
  • The investigation of new immuno-therapeutic approaches for virus-related tumors.
  •  
Quaglino Daniela

BIOLOGY AND PAHOLOGY OF THE EXTRACELLULAR MATRIX.

Ongoing research projects:

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  • On the role of ABCC6 and of modifiers genes in the phenotipic expression of  Pseudoxanthoma elasticum
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  • On the pathogenesis of ectopic calcification in aging and in genetic diseases
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  • Identification of markers  of  the severity of  ectopic calcification
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  • Development of a mineralized 3D scaffold for  cell cultures
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Recchia Alessandra

GENE TRANSFER AND GENE EDITING.

Recchia’s lab is focused on the design, development and validation of viral vectors for gene transfer in somatic cells. We have investigated the basic mechanisms of retroviral and lentiviral integration into the human genome and the influence of this event on the transcriptosome profiling of clinically relevant cell types. We are also interested in the development of new gene transfer technology based on the engineered nuclease and the Sleeping beauty Transposon.

Tarugi Patrizia

GENETICS, MOLECULAR PATHOLOGY AND PATHOPHYSIOLOGY OF MONOGENIC DISORDERS OF CHOLESTEROL AND PLASMA LIPOPROTEIN METABOLISM.

The more significant research lines are the following:

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  • Characterization of molecular bases and phenotypic expression  of monogenic and polygenic hypobetalipoproteinemias;
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  • Genetic determinants of Non Alcholic Liver Disease (NAFLD) associated with inherited dyslipidemias;
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  • Molecular bases of monogenic disorders of intracellular metabolism of cholesterol and triglycerides.
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Zappavigna Vincenzo

CONTROL OF GENE EXPRESSION IN EMBRYONIC DEVELOPMENT AND IN CANCER.

Research Topics:

My lab has since long been interested in the transcriptional control of developmental processes, especially those regulated by Hox genes and their co-factors. In the past years our main focus has shifted on the involvement of Hox transcription factors in cell cycle control and tumorigenesis, and on post-transcriptional regulatory mechanisms. The two main research topics we are currently exploring are:

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  • Study of the mechanisms underlying the perturbation of cell cycle control by NUP98-HOX and other NUP98 fusion oncoproteins;
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  • Study of the transcriptional regulation of miR196b and of the CDX2-miR196b-GATA6 regulatory network in colon carcinoma.
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To know more about our research projects click here.